Tilling Facility

TILLING genes in crop species, from data mining to characterisation of mutant phenotypes

TILLING work flow

Tools
- Metabolome
- Proteomics
- Sequencing
- Transcriptomics
- Gene cloning		 Data mining:
Candidate
Genes
 
 
 		 Reverse genetics
Platform
(TILLING)
 
 
 		 mutant
Characterisation
 
 
 
 		 Allelic varaiation studies
(ECOTILLING)
 
 
 

Tilling Platform

  • Tomato TILLING Platform: (Col. Bordeaux)
  • Rapseed TILLING Platform: (Col. Rennes)
  • Pea TILLING Platform: (Col. Dijon)
  • Medicago TILLING Platform: (Col. Rennes)
  • Melon TILLING Platform: (Col. Avignon)

Projects:

- GLIP FW6 2004 - 2008
- TRANSVIR: GNP05003G 2005 - 2007
- GENOTILL: GEN-079-089 2005 - 2007
- ECOTILLING: EU-Project 2005 - 2007
- EUSOL FW6: 2006 - 2010
- Endonucleases: INRA Transfert 2005
- Shoot branching ERAPG 2007 - 2010
- RiceTil: ANR 2005 - 2007
- Qualitylegseed: ANR 2006 - 2010
- MELRIP: ERAPG 2007 - 2010
- SNPEA: ANR 2006 - 2010
- TomSysBio BBSRC-ANR 2008 - 2010

Examples of tilled genes in tomato:

tomato tilling

Main achievements since 2004

  • - 2 patents on mutation detection
  • - 1 license accorded to SERIAL GENETICS for the sell of ENDO1
  • - TILLING licenses under signature with other companies
  • - 2 papers published,
  • - 2 submitted and more than 13 in preparation